植物组织培养技术
Class time： 2025-10-09 ~ 2026-12-31，Total 64 weeks
2.Gain proficiency in fundamental principles such as cell totipotency, clarify the internal logic of key knowledge including medium composition, explant treatment, and aseptic operation, and be able to link theory with practice by combining practical guidance.
3.Be familiar with the tissue culture characteristics and factory production standards of 20 plant species across three major categories: flowers, fruits/vegetables, and Chinese medicinal herbs.
4.Understand laboratory management standards, reagent safety protocols, industry quality certification requirements, and the causative mechanisms of common tissue culture issues (such as contamination and browning).
1.掌握植物有性/无性繁殖的特点，通过草莓脱毒、石斛快繁等实例理解组培 “高效、脱毒” 的核心优势；
2.精通细胞全能性等基础原理，明晰培养基组成、外植体处理、无菌操作等关键知识的内在逻辑，能结合实操指导关联理论与实践；
3.熟知花卉、果蔬、中草药三大品类共 20 种植物的组培特性与工厂化生产标准；
4.了解实验室管理规范、试剂安全规程、行业质量认证要求及组培常见问题（污染、褐化等）的成因机制。
This course serves as a core online boutique program for majors such as Horticulture and Modern Agricultural Technology. It is designed with a focus on "practical skills as the core and standardized procedures as the guideline," deeply aligning with the competency requirements of roles like Plant Tissue Culture Technician and Plant Factory Manager. The course is tailored for both self-directed learning and classroom instruction scenarios.
Built upon the four-dimensional teaching framework of "Theory-Virtual Practice-Hands-on Operation-Evaluation," the course spans a total of 108 planned class hours. It covers essential topics including plant propagation principles, tissue culture laboratory design, medium preparation, aseptic techniques, test-tube plantlet cultivation, and acclimatization/transplantation. Through 37 practical micro-lessons, 20 plant case studies, and supporting graphic resources, the course enables comprehensive skill development across the entire workflow.
The curriculum emphasizes tissue culture examples across 20 plant species in categories such as flowers, fruits/vegetables, and Chinese medicinal herbs. It integrates elements of craftsmanship and ideological-political education centered on technology-driven agricultural development. The course aims to cultivate students' comprehensive abilities to independently execute tissue culture production, troubleshoot issues, and optimize protocols. A dual-dimensional evaluation system—comprising "Process Assessment (60%) + Final Assessment (40%)"—ensures balanced emphasis on theoretical understanding and practical application, facilitating the transition from knowledge acquisition to job readiness.
本课程是园艺、现代农业技术等专业的核心网络精品课，以“实操能力为核心、规范流程为准则”，深度对接植物组培技术员、植物工厂管理员等岗位能力需求，适配自学与课堂学习双重场景。课程以“理-虚-实-评”四维教学体系为基础，总计划108课时，涵盖植物繁殖原理、组培实验室设计、培养基配制、无菌操作、试管苗培养与驯化移栽等核心内容，并通过37个实操微课、20种植物案例及配套图文资源，支撑全流程技能训练。
课程聚焦花卉、果蔬、中草药等20种植物组培实例，融合工匠精神与科技兴农思政元素，旨在培养学生独立完成组培生产、问题排查与方案优化的综合能力。采用“过程性考核（60%）+终结性考核（40%）”双维度评价体系，确保理论掌握与实操落地并重，实现从知识认知到岗位胜任的跨越。
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